Resources

Protocols

1. Rapid establishment of human colonic organoid knockout lines
2. Generation of gastric insulin-secreting (GINS) organoids from human stomach sample

Plasmids

For GINS organoids

1. pLenti-EF1a-Ngn3ER-PuroR-mCherry (Lentiviral backbone; EF1a promoter; Ngn3ER_2A_PuroR_2A_mCherry)
2. pLenti-CMV-PM (Lentiviral backbone; CMV promoter; Pdx1_2A_Mafa)
3. pLenti-TetO-PM (Lentiviral backbone; all-in-on TetOn system; Pdx1_2A_Mafa)

Codes

1. scRNA-seq analysis of GINS organoids (Nature Cell Biology, 2023)

Tweetorials

1. Restoring glucose homeostasis with stomach-derived human insulin-secreting organoids