Resources

Protocols

  1. Rapid establishment of human colonic organoid knockout lines
  2. Generation of gastric insulin-secreting (GINS) organoids from human stomach sample

Plasmids

For GINS organoids
  1. pLenti-EF1a-Ngn3ER-PuroR-mCherry (Lentiviral backbone; EF1a promoter; Ngn3ER_2A_PuroR_2A_mCherry)
  2. pLenti-CMV-PM (Lentiviral backbone; CMV promoter; Pdx1_2A_Mafa)
  3. pLenti-TetO-PM (Lentiviral backbone; all-in-on TetOn system; Pdx1_2A_Mafa)

Codes

  1. scRNA-seq analysis of GINS organoids (Nature Cell Biology, 2023)

Tweetorials

  1. Restoring glucose homeostasis with stomach-derived human insulin-secreting organoids

Weill Cornell Medicine Zhou Lab 1300 York Ave, A610 New York, NY 10065